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Agilent G1978B
Multimode Source for
6100 Series Single Quad
LC/MS
Set-Up Guide
Agilent Technologies
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Notices © Agilent Technologies, Inc. 2008 Warranty Safety Notices No part of this manual may be reproduced in The material contained in this docu- any form or by any means (including elec- ment is provided “as is,” and is sub- tronic storage and retrieval or translation CAUTION ject to being changed, without notice, into a foreign language) without prior agree- in future editions. Further, to the max- ment and written consent from Agilent A CAUTION notice denotes a haz- imum extent permitted
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In This Guide This guide explains how to install, maintain and troubleshoot your nanoelectospray ion source. Installation 1 This chapter tells you how to install the multimode ion source. Verification 2 This chapter tells you how to install the multimode ion source. Methods 3 This chapter describes basic operation and maintenance for the multimode ion source. Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 3
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4 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide
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Content 1 Installation 7 Installation 8 Step 1. Prepare to install 8 Step 2. Install the HV control PCA and cables 9 Changing Sources 13 To remove the multimode source 13 To convert from multimode to ESI, APCI or APPI 14 To convert from ESI, APCI or APPI to the multimode source 15 2 Verification 21 To verify sensitivity for 6120 Quad - G1978B Multimode, Positive SIM Modes 22 To verify sensitivity for 6130 and 6140 Quads - G1978B Multimode, Posit
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Contents 6 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide
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Agilent G1978B Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 1 Installation Installation 8 Step 1. Prepare to install 8 Step 2. Install the HV control PCA and cables 9 Changing Sources 13 To remove the multimode source 13 To convert from multimode to ESI, APCI or APPI 14 To convert from ESI, APCI or APPI to the multimode source 15 This chapter contains instructions to install the multimode source on a 6100 Series Single Quad LC/MS system, and also to
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1 Installation Installation Installation This section explains how to install the G1978B multimode source on a 6100 Series Single Quad LC/MS instrument. The G1978A multimode source is not supported on these instruments. The 6100 Series Single Quad LC/MS with multimode source is supported on ChemStation B.03.01 or higher, or the ChemStation B.01.03 with a patch. Step 1. Prepare to install The Multimode Enablement Kit, G1978-60451, is shipped with the multimode source. This kit needs to be in
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Installation 1 Step 2. Install the HV control PCA and cables � Valve BD-APCI supply, p/nG1960-60802 � Valve BD-APCI Needle Interlock, p/n G1960-60856 Figure 2 From left to right: G1946-80058, G1960-60802 and G1960-60856 Step 2. Install the HV control PCA and cables 1 Turn off the system power and remove the system power cord. Keep the power cord intact if the vacuum control switch box is used. The switch box is intended to keep the vacuum on while a CE works on the electronics. The switch b
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1 Installation Step 2. Install the HV control PCA and cables Figure 4 Connecting the valve PCA to the multimode HV power supply. 6 Install the APCI HV power supply. The APCI HV power supply is located at the end of the AUX Module. 7 Connect ribbon cable between valve PCA and Vcap/Vchamber power supply. Figure 5 Connecting the valve PCA to the Vcap/Vchamber power supply. 8 Connect the Vcap and Vchamber cables to the Vcap/Vchamber power supply. 10 Multimode Source for 6100 Series Single Quad L
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Installation 1 Step 2. Install the HV control PCA and cables Figure 6 Connecting the Vcap and Vchamber cables to the power supply. 9 Connect the long ribbon cable, p/n G1960-60802, from the APCI HV power supply to the valve PCA. Figure 7 Connecting the APCI HV power supply to the valve PCA. 10 Insert one end of the APCI Needle Interlock cable, G1960-60856, through the slot at the front of the system and then plug it to the APCI HV connector. Attach the other end to the chassis w
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1 Installation Step 2. Install the HV control PCA and cables Figure 9 Connecting the HV PCA to the chassis. 12 Close the AUX Module cover and reconnect all cables. 13 Install the multimode source to the system and connect all connectors. Figure 10 Installing the multimode source (left) and connecting all connectors. 14 Put back the side, top, front and CDS cover. 15 Plug the system power cord back on and turn the front switch on. The pump down process will start. 16 Start the ChemStation pr
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Installation 1 Changing Sources Changing Sources To remove the multimode source Do the following steps to remove the multimode source. 1 Set the source temperatures for vaporizer heater and drying gas heater to minimum values to cool the source. Use the Tune > Instrument > Edit Spray Chamber menu item to display the Edit Spray Chamber dialog box. Set the drying gas flow, nebulizer gas flow, drying gas temperature and the vaporizer temperature to minimum values. Do not touch the multimode
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1 Installation To convert from multimode to ESI, APCI or APPI 10 Place the source shipping cover on the source. To convert from multimode to ESI, APCI or APPI Never touch the source surfaces, especially when you analyze toxic substances or WARNING when you use toxic solvents. The source has several sharp pieces which can pierce your skin including the APCI corona needle, vaporizer sensor and counter current electrode. 1 If the source to be installed is an APPI source, disconnect the multimod
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Installation 1 To convert from ESI, APCI or APPI to the multimode source To convert from ESI, APCI or APPI to the multimode source If you are installing this source on this instrument for the first time, follow the steps in CAUTION “Installation” on page 7. 1 Switch to the MSD Tune view. 2 Select Instrument/Set Spray Chamber and set all gas flows and temperatures to 0. � Drying Gas (L/min) � Nebulizer Pressure (psig) � Drying Gas Temperature (°C) � Vaporizer Temperature (APCI source only) �
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1 Installation To convert from ESI, APCI or APPI to the multimode source Standard spray shield Capillary cap Figure 11 Standard spray shield and capillary cap for ESI or APCI 10 Remove the capillary cap. If needed, moisten a clean cloth with isopropyl alcohol and wipe the capillary cap. See Figure 12. Capillary cap Figure 12 Spray shield removed. 11 Place the capillary cap back on the capillary. 12 Install the new spray shield with field shaping electrodes. See Figure 13. 16 Multimode Source f
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Installation 1 To convert from ESI, APCI or APPI to the multimode source Figure 13 Multimode spray shield 13 Screw the multimode spray shield into the holder for the spray shield. See Figure 14. Field shaping electrode 9 o'clock position Field shaping electrode 6 o'clock position Figure 14 Multimode spray shield installed The field shaping electrodes should be in the nine o’clock and the six o’clock position. NOTE Loosen the end plate screws on each side to adjust the field shaping electrod
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1 Installation To convert from ESI, APCI or APPI to the multimode source Figure 15 Multimode Spray Chamber 15 Install the spray chamber on the spray chamber mount. I-Button Figure 16 Multimode source with I-Button 16 Install the nebulizer on the multimode source spray chamber. 18 Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide
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Installation 1 To convert from ESI, APCI or APPI to the multimode source Figure 17 No nebulizer on top of the multimode source 17 Connect the 1/8-inch nebulizer gas tubing from the LC/MS mainframe to the nebulizer gas fitting. See Figure 18. Sample Tubing Nebulizer zero dead volume Nebulizer gas fitting Figure 18 Nebulizer with gas tubing connected Multimode Source for 6100 Series Single Quad LC/MS Set-Up Guide 19
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1 Installation To convert from ESI, APCI or APPI to the multimode source 18 Connect the LC/MS sample tubing to the LC/MS diverter valve inlet filter. See Figure 19 on page 20. The LC/MS Liquid Chromatograph diverter valve is an integral part of the G1978B WARNING safety system. The LC mobile phase flow must always be connected to the diverter valve inlet filter. Never bypass the diverter valve and connect directly to the nebulizer. If the diverter valve is used in a manner not specified by
