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LeicaDM4000B
LeicaDM4000M
LeicaDM4500P
LeicaDM5000B
Operating Manual
Bedienungsanleitung
1
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Published September 2007 by: Herausgegeben September 2007 von: Leica Microsystems CMS GmbH Ernst-Leitz-Straße D-35578 Wetzlar (Germany) Responsible for contents: Verantwortlich für den Inhalt: Dr. Jasna Roeth, Stefan Motyka (Marketing CM, Compound Microscopy, Product Management) (Marketing CM, Compound Microscopy, Produktmanagement) Holger Grasse (Safety Officer according to MPG §30) (Sicherheitsbeauftragter nach MPG §30) In case of questions, please contact the hotline: Bei Fragen wenden Sie si
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Leica DM4000 B Leica DM4000 M Leica DM4500 P Leica DM5000 B Operating Manual 3
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Copyrights Copyrights All rights to this documentation are held by Leica Microsystems CMS GmbH. Reproduction of text or illustrations (in whole or in part) by print, photocopy, microfilm or other method (including electronic systems) is not allowed without express written permission from Leica Microsystems CMS GmbH. The term "Windows" may appear in the following text without further identification. It is, however, a registered trademark of Microsoft Corpora- tion. The names of companies and prod
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Contents Contents 1. Important notes about this manual ....... 7 7. Startup ......................................................... 35 7.1 Functional principle .................................. 35 2. Intended purpose of the microscope .... 8 7.2 Switching on the unit ................................ 38 7.3 The display 3. Safety notes ................................................ 9 (DM4000 B/4500 B/4000 M/4500 P) ......... 39 3.1 General safety notes................................. 9 7.4 T
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Contents 9. Contrast methods for 11. Troubleshooting ......................................... 74 Leica DM4000 B/DM4500 B/ DM4500 P/DM5000 B ................................ 60 12. Care of the microscope ........................... 77 9.1 Transmitted light ........................................ 60 12.1 Dust cover ................................................... 77 9.1.1 Bright field ......................................... 60 12.2 Cleaning...................................................
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1. Important notes about this manual 1. Important notes about this manual Caution! This operating manual contains important in- This operating manual is an essential com- structions and information for the operational ponent of the microscope, and must be read safety and maintenance of the microscope and carefully before the microscope is assem- accessories. It must therefore be kept safely for bled and put into operation. future reference. Text symbols, pictograms and their meanings: (1.2) Numb
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2. Intended purpose of the microscope 2. Intended purpose of the microscope The DM4000 – DM5000 microscopes to which these operating instructions belong, and which Caution! have the identifying letter B, are intended for biological routine and research applications. The manufacturer assumes no liability for This includes examining specimens taken from damage caused by, or any risks arising from the human body for the purpose of gaining infor- using the microscopes for other purposes mation about
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3. Safety notes 3. Safety notes 3.1 General safety notes Caution! This safety class 1 device was built and tested in accordance with the safety regulations for The devices and accessories described in electrical measuring, control, regulating and this operating manual have been tested for laboratory devices in accordance with safety and potential hazards. EN 61010-2-101:2002 The responsible Leica affiliate or the main EN 61010-1:2001 plant in Wetzlar, Germany, must be consult- IEC 1010-1:20
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3. Safety notes 3.2 Electrical safety ebq 100 supply unit* General specifications For indoor use only. Supply voltage: 90-250 V~ Leica CTR5000 electronics box (for DM5000 B) Frequency: 50-60 Hz For indoor use only. Power input: max. 155VA Supply voltage: 90-250 V~ Fuses: 2xT2A (IEC 127) Frequency: 50-60 Hz Ambient temperature: 10-36°C Power input: max. 290VA Relative humidity: max. 80% to 30°C Fuses: T6.3 A Overvoltage category: II (IEC 60127-2/3) Pollution degree: 2 Ambient temperature: 15-35°C
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3. Safety notes 3.3 Disposal Caution! Once the product has reached the end of its ser- vice life, please contact Leica Service or Sales Never use any fuses as replacements other about disposal. than those of the types and the current rat- ings listed here. Bypassing fuse holders is Please observe and comply with the national not permitted. and federal laws and regulations that are equiv- alent to EU guidelines such as WEEE. Caution! Note! The microscope’s electrical accessory com- ponents are no
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4. Overview of the instrument 4. Overview of the instrument Specification Leica DM4000 B Leica DM4000 M Leica DM5000 B Leica DM4500 P • Transmitted light: Contrast methods • Transmitted light: DM4000 M: BF, DF, PH, ICT, DM4000 B: BF, DF, PH, Pol Pol DM5000 B: and ICT (mot.) DM4500 P: BF, DF, PH, ICT, • Incident light: Fluorescent Pol (conoscopy) • Incident light: BF, DF, ICR, Pol, Fluo Transmitted light axis • Automatic illumination manager (mot. aperture diaphragm and field diaphragm, mot. inte
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4. Overview of the instrument Specification Leica DM4000 B Leica DM4000 M Leica DM5000 B Leica DM4500 P X/Y stage • Manual • Manual • Replaceable specimen stage • DM4000 M: • Coaxial drive length: 155 mm • Replaceable specimen stage • Coaxial drive length: 140 mm DM4500 P: • Replaceable Pol stage Tube • Manual or motorized (DM4500P: manual) • Optionally with one or two camera outputs • DM4500 P: conoscopy module (tube optics HC P1x/1.6x with Bertrand lens, encoded) Condenser • Motorized condense
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4. Overview of the instrument Specification Leica DM4000 B Leica DM4000 M Leica DM5000 B Leica DM4500 P Electronics box Only for the Leica DM5000 B: Leica CTR5000 Separate operating unit with a power supply for 100 W halogen lamps. See → p.10 (Electrical safety) 14
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4. Overview of the instrument 1 2 3 14 4 5 6 7 13 12 11 10 9 8 Fig. 1 Left side of the stand with the advanced AET22 ErgoTube 1 Eyepiece 8 Field diaphragm operating buttons 2 Eyepiece tube 9 Transmitted light / incident light switch 3 Tube 10 Aperture diaphragm operating buttons 4 Objective turret with objectives 11 Brightness adjustment buttons 5 Specimen stage with specimen holder 12 Focus wheel with coarse and fine adjustment 6 Condenser 13 Variable function keys (preset at the factory) 7 LCD
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4. Overview of the instrument 15 22 16 21 20 19 18 17 Fig. 2 Right side of the stand with the advanced ErgoTube AET22 15 Lamp housing for incident light 16 Lamp housing for transmitted light 17 Transmitted light filter, optional 18 Transmitted light filter, optional 19 Variable function keys (preset at the factory) 20 x/y coaxial drive, adjustable height 21 Handwheel for fine focus 22 Motorized filter block exchanger 16
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5. Unpacking the microscope 5. Unpacking the microscope The external ebq 100 supply unit* is delivered in The microscope is delivered in two packages. a separate package. The stand package contains the following com- For the Leica DM5000 B microscope: ponents: The Leica CTR5000 electronics box is delivered in a separate package. • Stand with integrated incident light axis and objective turret First, carefully remove all components from the transportation and packaging materials. • Specimen stage
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5. Unpacking the microscope Installation location Transport Work with the microscope should be performed For shipping or transporting the microscope in a dust-free room, which is free of oil vapors and its accessory components, the original and other chemical vapors, as well as extreme packaging should be used. humidity. At the workstation, large tempera- ture fluctuations, direct sunlight and vibrations As a precaution to prevent damage from vibra- should be avoided. These conditions can distor
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6. Assembly 6. Assembling the microscope The microscope components are logically as- 6.1 Specimen stage sembled in this order: ! Caution: • Specimen stage • Condenser with condenser head Never install objectives before assembling the • Tube stage. • Eyepieces • Objectives • Place the specimen holder on the stage and • Lamp housings with light sources fasten it with the two screws (3.1). • Equipment for the incident light turret disk* • Using the condenser height adjuster (3.2), turn Only a
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6. Assembly • From above, set the stage clamp onto the • Only for DM4500 P: dovetail guide (4.2) and push the stage down- wards until the upper end of the dovetail Pol attachable mechanical stage* guide is tightly fastened to the upper end of Adjust the attachable mechanical stage so the stage clamp. that the fastening screw is visible below the holes (4a.1). Set the attachable mechanical • Firmly tighten the stage clamp (4.1). stage in the guide holes on the rotating stage and tighten the faste
