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Leica DM4000 B
Leica DM4000 M
Leica DM5000 B
Operating Manual
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Published 2003 by: Leica Microsystems Wetzlar GmbH Ernst-Leitz-Straße D-35578 Wetzlar (Germany) Responsible for contents: Katja Peter, Karin Schwab Phone +49(0)6441-292261 Marketing CM, Compound Microscopy, Product Management Fax +49(0)6441-292255 In case of questions, please contact: E-mail: MQM-Hotline@leica-microsystems.com 2
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Leica DM4000 B Leica DM4000 M Leica DM5000 B Operating Manual 3
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Copyrights Copyrights All rights to this documentation are held by Leica Microsystems Wetzlar GmbH. Reproduction of text or illustrations (in whole or in part) by print, photocopy, microfilm or other methods (includ- ing electronic systems) is not allowed without express written permission from Leica Microsystems Wetzlar GmbH. The term "Windows" can be used in the following text without further identification. It is a registered trademark of the Microsoft Corporation. Otherwise, no inference wit
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Contents Contents 1. Important Notes about this Manual ..... 7 6. Startup ........................................................ 31 6.1 Functional Principle.................................. 31 6.2 Switching on the Microscope ................ 34 2. Safety Notes .............................................. 8 6.3 The Display 2.1. General Safety Notes ............................... 8 (Leica DM4000 B/DM4000 M) ................. 35 2.2. Electrical Safety ........................................
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Contents 8.2 Fluorescence ............................................. 57 9. Imaging Procedure for 12. Essential Leica DM4000 M ....................................... 58 Wear and Spare Parts ............................. 66 9.1 Incident Light ............................................. 58 9.1.1 Bright Field ...................................... 58 13. Abbreviations and Pictograms .............. 67 9.1.2 Dark Field......................................... 58 9.1.3 Polarization ..............
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1. Important Notes about this Manual 1. Important Notes about this Manual Caution! This operating manual contains important in- This operating manual is an essential com- structions and information for the operational ponent of the microscope, and must be read safety and maintenance of the microscope and carefully before the microscope is put into accessories. Therefore, it must be kept and operation or used. taken care of. Text symbols and their meanings: (1.2) Numbers in parentheses, such as "
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2. Safety Notes 2. Safety Notes 2.2 Electrical Safety 2.1 General Safety Notes General specifications This safety class 1 device is constructed and tested in accordance with EN 61010-1/IEC 1010-1, Leica CTR5000 electronics box (for DM5000 B) safety regulations for electrical measuring, con- For indoor use only. trol, and laboratory devices. Supply voltage: 90-250 V~ Frequency: 50-60 Hz Power input: max. 290 VA Caution! Fuses: T6,3 A (IEC 60127-2/3) In order to maintain this condition and to en-
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2. Safety Notes Supply unit ebq 100 Caution! For indoor use only. Supply voltage: 90-250 V~ Never use any fuses as replacements other Frequency: 50-60 Hz than those of the types and the current rat- Power input: max. 155 VA ings listed here. Using patched fuses or Fuses: 2xT2A (IEC 127) bridging the fuse holder is not permitted. Ambient temperature: 15-35°C Relative humidity: max. 80% to 30°C Overvoltage category: II Caution! Pollution degree: 2 (see enclosed manual) The microscope’s electrical
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3. Overview of the Instrument 3. Overview of the Instrument Leica DM4000 B / DM5000 B Specification Leica DM4000 M • transmitted light: BF, DF, PH, Imaging Procedure • transmitted BF, DF, PH Pol (DM5000 B also ICT) light: ICT, Pol • incident light: fluorescence • incident light: BF, DF, ICR, Pol Transmitted Light Axis • automatic Illumination Manager (motorized aperture diaphragm and field diaphragm, motorized intensity control) • automatic Constant Color Intensity Control (CCIC) • motorized sh
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3. Overview of the Instrument Specification Leica DM4000 B / DM5000 B Leica DM4000 M • motorized condenser head Condenser • motorized condenser turret for light rings, DF stop, DIC prisms • optional polarizer integrated and motorized • automatic Köhler Illumination Magnification Changer • manual • manual • absolute coded • absolute coded • 1x; 1.25x; 1.6x • 1x; 1.5x; 2x • operating buttons for all motorized microscope functions Control Panels • additional variable function keys • focusing knobs
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3. Overview of the Instrument 1 2 3 14 4 5 6 7 13 12 11 10 9 8 Fig. 1 Leica DM4000 M left side of the stand with AET22 advanced ergotube 1 Eyepiece 8 Function keys field diaphragm 2 Eyepiece tube 9 Transmitted light/incident light switch 3 Tube 10 Function keys aperture diaphragm 4 Objective nosepiece with objectives 11 Function keys: Light intensity 5 Specimen stage with specimen holder 12 Focus dial with coarse and fine adjustment 6 Condenser 13 Variable function keys (factory pre-assigned
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3. Overview of the Instrument 15 22 16 21 20 19 18 17 Fig. 2 Leica DM4000 B right side of the stand with Advanced Ergotube AET22 15 Lamp housing for incident light 16 Lamp housing for transmitted light 17 Transmitted light filter, optional 18 Transmitted light filter, optional 19 Variable function keys (factory pre-assigned) 20 X/Y coaxial drive, height adjustable 21 Focus fine adjustment 22 Motorized filter cube exchanger 13
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4. Unpacking the Microscope 4. Unpacking the Microscope The external ebq 100 supply unit* is delivered in The device is delivered in two boxes. separate packaging. The stand box contains the following compo- For the Leica DM5000 B microscope: nents: The CTR5000 electronics box is also delivered in separate packaging. • Stand with integrated incident light axis and objective nosepiece First, carefully remove all components from the transportation and packaging materials. • Specimen stage with sta
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4. Unpacking the Microscope Installation location Transport Work with the microscope should be performed For shipping or transporting the microscope in a dust-free room, which is free of oil vapors and its accessory components, the original and other chemical vapors, as well as extreme packaging should be used. humidity. At the workplace, large temperature fluctuations, direct sunlight and vibrations As a precaution to prevent damage from vibra- should be avoided. These conditions can distort ti
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5. Assembly 5. Assembling the Microscope The microscope components are logically as- When using intermediate systems and optical accessories, the sequence may vary. sembled in this order: In this case, read Chapter, "5.10 Optional accessories" → p. 29 • Stage • Condenser • Tube • Eyepieces • Objectives • Light sources • Filter cubes/reflectors* Only a few commonly used screwdrivers and keys are necessary for assembly, which are in- cluded in the delivery package. 16
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5. Assembly 5.1 Stage • From above, set the stage clamp onto the dovetail guide (4.2) and push the stage down- wards until the upper end of the dovetail guide Caution: ! is tightly fastened to the upper end of the stage clamp. Before assembling the stage, make sure no ob- jectives are installed! • Firmly tighten the stage clamp (4.1). • Place the specimen holder on the stage and fasten it with the two screws (3.1). • Using the condenser height adjuster (3.2), turn Note: the condenser holder comp
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5. Assembly 5.2 Condenser Note: The condenser must be centered before using • Using the condenser height adjuster (5.4), turn the microscope. the condenser holder (5.1) completely down- Köhler illumination p. 37. → wards. • Unscrew the clamping screw for the con- Fig. 6 Underside of condenser denser (5.3) far enough so that the condenser 1 1 Orientation pin can be inserted from the front. • From the front, insert the condenser into the condenser holder as far as it will go. On the underside
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5. Assembly Fig. 9 Fastening the tube 5.3 Tube and Eyepieces 1 Clamping screw The tube is mounted to the stand either directly or with the use of intermediate modules. It is fastened in place with the side clamping screw (9.1). 1 • Loosen the clamping screw (9.1). • Insert the tube in the circular receptacle (dovetail ring). • Retighten the clamping screw (9.1). • Only for the MBDT motorized tube: Connect the tube to the stand with the con- nector socket (10.1). Fig. 10 Motorized tube connection
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5. Assembly Fig. 12 5.5 Light Sources for the Transmitted Light Axis Lamp housing 107/2 Releasing the fastening screw Caution: Be sure that the lamp housing is discon- nected from the power supply. Unplug the power plug and the power supply during as- sembly. 107/2 Lamp Housing This lamp housing is used with a 12V 100W halo- Fig. 13 gen lamp, which is already mounted. 1 Lamp housing 107/2, In case the lamp has to be removed: opened 1 Mount with • Remove the fastener screw on the housing halogen
