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Leica DM IRB
Instructions
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Issued in 1998 by: Leica Microsystems Wetzlar GmbH Ernst-Leitz-Strasse D-35578 Wetzlar (Germany) Responsible for contents: Marketing MQM, product management, Tel. +49 (0) 64 41-29 25 19 Fax +49 (0) 64 41-29 22 55 2
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Leica DM IRB Instructions 3
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Copyrights All rights to this documentation and the software it describes are owned by Leica Microsystems Wetzlar GmbH. Copying of text and illustrations – in full or in part – by printing, photostat, microfilm or other techniques, in- cluding electronic systems, is only permitted subject to the express written consent of Leica Microsystems Wetzlar GmbH. The information contained in the following documentation represents the latest stage of technology and knowledge. We have composed the texts an
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Contents Important notes on this manual ....................... 7 Assembly .............................................................. 17 Assembly tools .................................................... 17 General safety information ............................... 8 Assembly of the transmitted light illumination column.............. 17 Intended application .......................................... 10 Assembly of condensers ................................... 18 Assembly of IC condenser pr
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Contents Learn mode .......................................................... 43 Operation Installing the objective prisms ......................... 43 of transmitted light interference contrast ..... 78 Learning the IC objective prisms ..................... 43 Installing the objectives .................................... 44 Operation of incident light fluorescence ...... 81 Parfocality ............................................................ 47 Dry objectives .............................
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Important notes on this manual This manual is an integral part of the Leica The manual is multi-lingual. Due to the spiral DM IRB microscope and must be read carefully binding you can turn the language version you before you start using the microscope. want to the front. This manual contains important instructions and The DMIR is available both as a life sciences information on the operating safety and microscope and as a metallographic/industrial maintenance of the system. It must therefore be
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General safety information This instrument of safety class 1 has been built Make sure that only fuses of the specified type and tested according to EN 61010-1/IEC 1010-1/ and rating are used as replacements. It is VDE 0411-1, safety standards for electrical forbidden to use mended fuses or to short- measurement, control and laboratory equip- circuit the fuse holder. ment. n. b.: n. b.: The instruments and accessories described To keep the microscope in this safe condition, in this manual have
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n. b.: n. b.: The electric accessories of the microscope Protect the microscope from major tem- are not waterproof. If water gets inside them, perature fluctuations. These may lead to it may cause electrical shock. condensation which can damage the electric Do not put the microscope and its acces- and optical components. sories too near a water supply or anywhere else where water may get inside them. n. b.: n. b.: Avoid skin contact when using immersion oil! Ask the supplier for a safety info
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Intended application The new DMIRB is the logical further development of the successful inverted research microscope from Leica. It is used for examinations of cells and tissue, for micromanipulation and microinjection tech- niques all the way through to microdissection or confocal microscopy. The DM IRB has universal application potential, incorporating all the contrasting techniques of brightfield, darkfield, phase contrast, DIC, fluorescence and Hoffmann modulation contrast, which are all eas
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The microscope and its components Key subassemblies The following views of the whole microscope show and name important subassemblies of the microscope and its accessories. Fig. 1 – 2 1 Binocular phototube, 2 Eyepiece adapter tube, 3 Eyepieces, 4 Tube mount (tube change interface), 5 Tube port for photo/TV connection, 6 Beamsplitter switch, 7 Mains switch, 8 Brightness adjustment, 9 Lateral TV port, 10 Coaxial coarse and fine drive, 11 Fluorescence module, 12 ICT prism adjustment, 13 Sextuple ob
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The stand The stand with bottom port cannot be equipped with an SLR front port. This variant is only There are 5 basic versions of the DM IRB stand, produced at a customer’s specific request. which allow over 50 microscope variants to be configured. The electronic stand additionally offers a motorised objective nosepiece, electronic These 5 basic versions are: focusing, IC objective prism coding, LCD display – Manual or electronic stand of microscope functions and (optional) – With or without in
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Brightness adjustment Aperture diaphragm A 12 V 100 W transformer is built into the stand The aperture diaphragm determines the for stepless regulation of brightness with the resolution, field depth and contrast of the brightness control. microscope image. The best resolution is achieved when the apertures of the objectives Coarse and fine control and the condenser are roughly the same. The coarse and fine focus control allows fast and precise focusing of the microscope image. n. b.: Focusing i
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Specimen stages and accessories Transmitted light illumination unit The specimen stage supports the specimens The transmitted light illumination unit consists of that are to be examined through the mi- the lamphousing and the transmitted light croscope. Several options are available to illumination column. The transmitted light accommodate the wide variety of specimens lamphousing comprises a precentred 12 V 100 W examined, such as object guides, extension halogen lamp and a filter module for th
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Installation site The microscope should be used in a dust-free room which is free of oil and chemical fumes n. b.: and extreme humidity. Also, the workplace should not be exposed to major temperature Lamphousings* and power units* must be fluctuations, direct sunlight or vibrations. These placed at least 10 cm away from the wall and may impair measurements or photographs of the from flammable objects. microscope image. Ambient conditions: Temperature 10 – 36 °C Relative humidity 0 – 80 % to 30
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Unpacking Installation c First take all the components out of the Please compare the delivery carefully with the transport and packing material. packing note, delivery note or invoice. We c Put the basic DM IRB stand on a desk which strongly recommend that you keep a copy of has enough room for it. these documents with the manual, so that you have information on the time and scope of the delivery later when ordering more equipment or when the microscope is serviced. Make sure n. b.: that no smal
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Assembly Assembly tools Assembly of the transmitted light illumination column Installation and assembly of the microscope should preferably be carried out together with a Wipe off the interface surface (4.3) with a dry member of Leica sales or service staff. cloth. Tilt the illumination column (4.1) slightly to Only a few ordinary screwdrivers are required the back and insert so that the pin (4.2) engages for assembly, and these are supplied with the in the groove of the interface surface (4.4).
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The lamphousing for transmitted light Assembly of condensers illumination for 12V 100W halogen lamps with The technical description of the condensers can single-lens aspherical collector and heat be found in the chapter “Technical description”. protection filter is an integral part of the All condensers of the Leica DMIRB are transmitted light illumination column. The halo- equipped with a 6-position rotating disc (6.2 and gen lamp is preassembled. The chapter on 8.3) and can be individually fit
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Insert the light rings for Phaco (identified by the – Using the centring keys, screw the centring code numbers 0, 1, 2, 3 and the intercept screws back in until they no longer protrude distance S of the corresponding condenser top, over the outer edge of the disc. e. g. 2 S1) and the DF diaphragm (identified by D – Fit IC condenser prisms if appropriate (see for darkfield and the intercept distance S of the assembly of ICT objective prisms). corresponding condenser top, e.g. DS1, see – Insert th
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5 1 6 4 3 Fig. 8 Condenser 0.53 S23 2 1 Condenser base, 2 Condenser top 0.53 S23 (inter- changeable), 3 Condenser disc, 4 Aperture diaphragm, 5 Filter holder, 6 Dovetail guide 1 3 2 Fig. 9 Condenser tops for condenser base (8.1) 4 1 Condenser top 0.53 S23, 2 Condenser top 0.90 S1, 3 Condenser top P 1.40 OIL S1, 4 Spacer ring for assembling 9.2 and 9.3 6 1 11 10 2 9 Fig. 10 6-position condenser disc, empty 4 3 1 Condenser disc with slots for light rings and IC condenser nd prisms, 2 Guide groo
