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Leica M165 FC
Leica M205 FA
User Manual
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General Instructions Safety concept Use in clean rooms Servicing ρ Before using your microscope for the first time, The Leica M series can be used in clean rooms Repairs may only be carried out by Leica please read the "Safety concept" brochure without any problems. Microsystems-trained service technicians. included with your instrument. It contains addi- Only original Leica Microsystems spare tional information about handling and care. Cleaning parts may be used. ρ Do not use any unsuita
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Important Safety Notes User Manual You can combine individual system articles Special manuals The individual modules of the Leica M stereo- with articles from external suppliers (e.g. cold Special manuals are provided for a number of microscopy series include an interactive light sources, etc.). Please read the user manual accessories: CD-ROM with all relevant user manuals in and the safety requirements of the supplier. ρ 20 other languages. Keep it in a safe place, User Manual M2-216-1 for la
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Symbols Used Warning of a danger Danger due to hot surface This symbol indicates especially impor- This symbol warns against touching • tant information that must be read and accessible hot surfaces, e.g. those of complied with. light bulbs. Failure to comply can cause the following: ρ Hazards to personnel Important information ρ Functional disturbances or damaged instru- This symbol indicates additional infor- ments mation or explanations that intend to provide clarity. Warning of hazar
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Safety Instructions Description are outside of the scope of this manual. Responsibilities of person in charge of The individual modules fulfill the highest instrument requirements for observation and documenta- Unauthorized alterations to the instrument or ▶ Refer to "Safety Concept" booklet tion of Leica stereomicroscopes of the M series. noncompliant use shall void all rights to any warranty claims. Ensure that: ρ Intended use The M series stereomicroscopes and ▶ Refer to "Safety Concept"
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Safety Instructions (cont'd.) Repairs, service work Integration in third-party products this reason, appropriate measures for reduction ▶ Refer to "Safety Concept" booklet ▶ Refer to "Safety Concept" booklet of the workload must be taken: ρ Only original Leica Microsystems spare ρ Optimal arrangement of workplace, work parts may be used. Disposal assignments and work flow (changing tasks ▶ Refer to "Safety Concept" booklet frequently). ρ Before opening the instruments, switch off the powe
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Safety Instructions (cont'd.) Direct contact with eyepieces is a poten- Light sources: Safety regulations Warning • tial transmission method for bacterial Protective measures of the manufacturer: UV radiation could damage the eyes. ρ and viral infections of the eye. UV protection screen in front of the speci- • Therefore: men plane prevents the user from looking directly into the UV rays. ρ The risk can be kept to a minimum by using Never look into the light spot on the speci- personal eyepi
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Safety Instructions (cont'd.) Supply unit Lamp housing Mercury lamp ρ Always unplug the supply unit from the power Never open the lamp housing while the Follow the user manual and safety instruc- supply: • lamp is switched on. Risk of explosion, tions provided by the lamp manufacturer, ρ When installing and disassembling the UV exposure, blinding! and, in particular, the section on how to lamp housing proceed if a lamp breaks and releases mercury. Before opening the lamp housing, allow ρ
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Contents General Instructions 2 Transmitted-light Base TL ST 30 Important Safety Notes 3 FluoCombi III™ – Preparations 31 Symbols Used 4 FluoCombi III™ – Assembly 32 Safety Instructions 5 FluoCombi III™ – Adjusting the Parfocality 34 FluoCombi III™ – Centering the Objectives 36 The Leica M Series for Fluorescence FluoCombi III™ – Filter and UV Protection Screen 37 Congratulations! 13 Transmitted-light Base TL BFDF: Before First Use 38 A Step Towards Infinity 14 Transmitted-light Base TL BFDF 39
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Quick Start Guide Microscope Carrier The Fastest Route to Success 64 The Objective Nosepiece 86 Overview of an M Series Microscope 65 Objectives andOptical Accessories The Correct Interpupillary Distance 66 The Different Types of Objectives 88 Using the Eyepieces 67 Focusing 68 Bases Adjusting the Resistance of the Focus Drive 69 Leica TL ST Transmitted-light Base: Controls 90 Changing Magnification (Zoom) 70 Leica TL ST Transmitted-light Base: Operation 91 Ratchet Steps and Magnification Levels
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System Illumination Dimensional Drawings Leica LED5000 MCI™ 106 Leica M165 FC 129 Leica LED5000 RL 108 Leica M205 FA 132 Leica EL6000 – About the Instrument 109 Leica LED5000 MCI 135 Leica EL 6000 – Operation 111 Leica LED5000 RL 136 Leica EL 6000 – Troubleshooting 112 MATS TL 137 Cleaning and Maintenance 113 Specifications Fluorescence Leica M165 FC / Leica M205 FA 139 Filter Changer 115 Leica EL6000 – Specifications 143 FIM – Fluorescence Intensity Manager 116 Leica TL ST Transmitted-light Bas
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The Leica M Series for Fluorescence Leica M-series User Manual 12
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Congratulations! Congratulations on obtaining your new Leica M series stereomicroscope. Though the reliability and robustness of Leica stereomicroscopes is We are convinced it will exceed your expectations, as never before have legendary, like any high-tech product, the Leica M series requires a certain we applied our decades of experience in the areas of optics, mechanical degree of care and attention. Therefore, we recommend that you read engineering and ergonomics in such an uncompromising
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A Step Towards Infinity Ever since their introduction by Horatio S. Limits are made to be broken Greenough, stereomicroscopes have worked The Leica M205 FA is the world's first fluores- according to the optical principles based cence stereomicroscope with a zoom range primarily on Ernst Abbe's research. For over a of 20.5:1. This accomplishment, however, was century, ingenious optics designers and engi- not enough for Leica's engineers. With the new neers have worked to push magnification, re
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The Electronics: Comfort, Convenience and Safety for Your Experiments Never before have electronics been used as Reliability for your experiments diaphragm and more and transmits the infor- extensively in a Leica series as in the new M Exact reproducibility of results is an increas- mation to the Leica LAS software. Thus you series lineup. Optics carrier, stand, base and ingly common demand, particularly in research always know the conditions under which an illuminator are all connected using
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The Modular Design: Everything is Relative The Leica M series provides maximum flexibil- Despite this, you will notice that the controls Have a special request? Let us know! ity in choosing equipment, thanks primarily to and individual components do not differ signif- Leica Microsystems enjoys an exceptional repu- the modular configuration and the compatibil- icantly. Whichever configuration you choose, tation when it comes to devising customer- ity that Leica has painstakingly maintained for yo
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Maximum Compatibility Leica engineers were careful to ensure that the Tubes new Leica M series—like its predecessors— The interface between the optics carrier and the remains compatible with existing series. This tube has remained the same, so existing tubes means that objectives, bases, tubes and so on fit the new M series. The new tubes are designed can be reused. for eyepieces with field number 23, while the predecessor models were only designed for field Objectives number 21, resulting
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On We Go If your new Leica microscope has already been If, on the other hand, you are assembling For everything you need to know about the assembled and commissioned by your Leica the microscope yourself, continue with the correct use of fluorescence-related parts, refer to consultant, click here to skip through the instal- "Assembly" chapter, which begins on Page 19. Page 114. lation instructions and go directly to the Quick Start Guide on Page 63. Leica M-series User Manual 18
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Assembly Leica M-series User Manual 19
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Assembling the Focusing Column for MDG Bases The first step is to connect the focusing column Assembling the column adapter Assembling the focusing column of the M series to the corresponding base. 1. Securely install the column adapter on the 2. Securely screw the focusing column to the column using the four included screws. base using the six included screws. Tools used ρ Hex socket screwdriver, 3 mm Leica M-series User Manual 20
