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402 Dilutor Dispenser
User’s Guide
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402 Dilutor Dispenser User’s Guide LT801310/C - ©2003 Gilson SAS All rights reserved November 2003
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Contents Table of Contents 0 Safety 1 Introduction Notational Conventions .................................................................. 1-2 Overview .......................................................................................... 1-3 Unpacking ...................................................................................... 1-5 Warranty ......................................................................................... 1-6 Customer Service ..............................
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Upgrading to Dual-Syringe Model ........................................... 3-10 Removing the Cover ............................................................. 3-11 Connecting the Flat Cable.................................................... 3-11 Inserting the Module ............................................................ 3-11 4 Operation - Standard Use Essential Steps .................................................................................. 4-2 Standard Parts...................
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Changing the Piston Syringes and Piston Noses .................... 6-7 Changing the 100 and 250 µL Piston Noses ...................... 6-7 Changing the 500 µL Piston Noses ...................................... 6-9 Changing the 1 mL Piston Noses ....................................... 6-10 Changing the 25 mL Piston Noses ..................................... 6-11 Changing the 5 mL and 10 mL Piston Noses................... 6-11 7 Troubleshooting Mechanical Problems ...............................
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Safety 0 Safety Read this section carefully before installing and operating the instrument. For safe and correct use of the instrument, it is essential that both operating and service personnel follow generally accepted safety procedures as well as the safety instructions given in this document, the 402 Dilutor-Dispenser User’s Guide. The instrument described in this document is a syringe operated pump for transfer- ring liquids. It should only be used in the laboratory or similar indoor environ
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Safety 1 1 Individual components: This instrument must not be sterilized, using an parts of the valve, the Tee autoclave, or any other mechanical device. When junction, and the syringes you need to clean an instrument, use one of the (except for the 25 mL syringe) three following methods: may be autoclaved after 1 - a clean dry cloth, dismounting (121°C, 0.1 MPa, 2 - a cloth dampened with water, 20 min). 3 - a cloth dampened with soapy water. If a cloth dampened with soapy water is used to clea
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Introduction 1 The following chapter describes the instrument, the service and warranty conditions. 1-1
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Introduction 1 Notational Conventions The conventions in this User’s Guide are as follows. Used in the stand-alone mode, this instrument is referred to as ‘402 Dilutor-Dispenser’ or simply 402. Specific keys to press on the Gilson Keypad are represented in bold capitals, for example: ‘key in the value and press ENTER’; where ENTER is a key on the keypad. For a soft-key that must be pressed, the function of that key is represented in bold, for example: ‘Press Prime to prime the pump’. The Gilson
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Overview Introduction 1 Overview The 402 Dilutor-Dispenser, is used to transfer liquids between vials according to user defined protocols (Methods). You program the 402 Dilutor-Dispenser via the keypad, which includes an LCD screen, for communication purposes. You may store a total of 99 linkable Methods using the keypad. Each Method consists of one or more of the following tasks: Dilute, Dispense, Mix, Pipette, and Rinse. In addition three functions for direct manipulation are available: Primin
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Introduction 1 Good Laboratory Practice (GLP) is facilitated by the following: codes to protect the program files against unau- thorized modification, counters for the number of syringe and valve operating-cycles, checking protocol for the hydraulic specifica- tions (IQ/OQ procedures), correction of the syringe-piston stroke to com- pensate for environmental factors (temperature, viscosity, etc.). 1-4 Overview
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Unpacking Introduction 1 Unpacking Your 402 Dilutor-Dispenser is shipped in a box together with its various accessories. The original box and packing material should be kept in case the equipment has to be returned to your Gilson distributor. You should check off the parts received in the box against the parts list (refer to Appendix A), at the time that you receive the shipment, even if the 402 is not to be used immediately. Your Gilson distribu- tor should be notified immediately of any incons
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Introduction 1 Warranty If the 402 Dilutor-Dispenser does not appear to function correctly, first verify the electrical connec- tions are correct and that the instrument is switched ON. Contact your Gilson distributor for technical advice or possibly a service visit. Any service required will be given within the warranty conditions assured by your Gilson distributor. 1-6 Warranty
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Customer Service Introduction 1 Customer Service Gilson and its worldwide network of authorized distributors provide you with four types of assist- ance: sales, technical, applications and service. Customer Service personnel are able to serve you more efficiently if you provide the following information: •The serial number and model number of the equipment involved. •The installation procedure you used. • A concise list of the symptoms. •A list of operating procedures and conditions you were usi
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Introduction 1 1-8
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Description 2 The following chapter describes the mechanics and hydraulics of the Dilutor-Dispenser. Two models of 402 Dilutor-Dispenser are available: Single-syringe and Dual-syringe. Each model comes with a keypad, a probe, and accessories. You order and fit the syringe(s), from those described on page 2-6, most suited to your application. Your 402 is delivered with the appropriate modules fitted. If later, you decide to convert a Single-syringe model into a Dual-syringe model, you must order
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Description 2 Single-syringe Model The Single-syringe model has a Valve module at the left, and a blanking plate at the right. The Valve module includes one motor to operate the syringe and another motor to switch the valve. It is a liquid transfer station that can be used to deliver up to 25 mL in one stroke, and down to 1 µL (typical value), with high accuracy. Bottle Clip for Tubing Tubing Clip (Self-adhesive) Cable Support Valve Module ON/OFF indicator Clamp for Probe Front Panel The front p
