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Living up to Life
User Manual
Leica M125 B
Leica M165 C B
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Copyright All rights to this documentation are held by Leica Microsystems CMS GmbH. Reproduction of text or illustrations (in whole or in part) by print, photocopy, microfilm or other method (including electronic systems) is not allowed without express written permission from Leica Microsystems CMS GmbH. The instructions contained in the following documentation reflect state-of-the-art tech- nology. We have compiled the texts and illus- trations as accurately as possible. Still, we are R
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Function of the Microscopes The Leica M125 B and Leica M165 C B micro- other purposes than those for which they are instruments in accordance with EN 60601-1. If a microscope is electrically connected to a medi- scopes, for which this User Manual has been intended or not using them within the speci- cal instrument in accordance with 60601-1, the written, are designed for routine examinations fications of Leica Microsystems CMS GmbH. requirements listed in EN 60601-1-1 shall apply. of cell a
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General Notes Use in clean rooms ★ The cleaning of glass surfaces and objec- Servicing The Leica M series can be used in clean rooms tives in particular should be carried out ★ Repairs may only be carried out by Leica without any problems. exclusively as outlined in the brochure Microsystems-trained service technicians. "Cleaning of Microscope Optics". The infor- Only original Leica Microsystems spare Cleaning mation can be downloaded at: parts may be used. ★ Do not use any unsuitable cleani
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Important Safety Notes User Manual Before installing, operating or using the instru- We guarantee the quality of our products. This User Manual describes the special func- ments, read the user manuals listed above. In Our guarantee covers all faults in materials tions of the individual modules of the Leica M particular, please follow all safety instructions. and manufacture. It does not, however, cover stereomicroscopy series and contains impor- damage resulting from careless or improper tan
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Symbols Used Warning! Safety hazard! Danger due to hot surface. Disposal This symbol indicates especially impor- This symbol warns against touching hot Notes on how to dispose on the micro- • tant information that is mandatory to surfaces, e.g. those of light bulbs. scope, its components and expend- read and observe. ables. Failure to comply can cause the following: Important information ★ Hazards to personnel This symbol indicates additional infor- China RoHS 50 year EFUP (Environ- ★ Function
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Safety Instructions Description and mechanical parts unless expressly Place of use ★ The individual modules fulfill the highest instructed to do so in the instructions. ★ Only use the instruments in closed, dust requirements for observation and docu- free rooms and between +10 °C and mentation of Leica stereomicroscopes of +40 °C. Protect the devices from oil, chemi- the M series. The instruments and accessories described in cals and extreme humidity. If using the this User Manual have been t
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Safety Instructions (continued) Responsibilities of person in charge of ★ Before opening the instruments, switch off Integration in third-party products instrument the power and unplug the power cable. ★ When installing Leica products into third- ★ These Safety Instructions must be available party products, the manufacturer of the at the workplace. ★ Avoid contact with powered electrical complete system or its dealer is responsible circuits, which can lead to injury. for following all applicab
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Safety Instructions (continued) Legal regulations assignments and work flow (changing tasks Careful handling ★ Observe the generally applicable statu- frequently). Thorough training of the person- ★ Exercise particular care when setting up tory and country-specific regulations for nel, giving consideration to ergonomic and the instruments. If it is specified that two or accident prevention and environmental organizational aspects. more people are required for setup, compli- protection. The erg
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Safety Instructions (continued) Light sources: safety regulations External power supply for the focusing External power supply for TL5000 Ergo ★ Light sources pose a potential risk from column Permitted power supply: glare. Therefore, lamps have to be operated (Leica M165 C B) SINPRO SPU130-110 in closed housings and in installed condi- Permitted power supply: tion. SINPRO SPU46-110 Specifications: Input: 100-240 V AC ★ Never look directly into the beam path Specifications: 47-63 Hz (blinding
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Contents Copyright 2 Quick Start Guide Function of the Microscopes 3 The Fastest Route to Success 29 General Notes 4 Overview of an M Series Microscope 30 Important Safety Notes 5 The correct interpupillary distance 31 Symbols Used 6 Using the Eyepieces 32 Safety Instructions 7Focusing 33 Adjusting the Resistance of the Focus Drive 34 The Leica M Series Changing the Magnification (Zoom) 35 Congratulations! 14 Click Stops and Magnifications 36 A Step Towards Infinity 15 Parfocality: More Comfort
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Optical Accessories The Different Types of Objectives 46 Bases Leica TL5000 Ergo Transmitted Light Base: Controls 48 Leica TL5000 Ergo Transmitted Light Base: Operation 49 Technical Data Leica TL5000 Ergo Transmitted Light Base 52 Appendix Calculating the Total Magnification and Field of View Diameter 54 Care, Maintenance, Contact Persons 55 Leica M series User Manual 12
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The Leica M Series Leica M series User Manual 13
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Congratulations! Congratulations on obtaining your new Leica M series stereomicroscope. Though the reliability and robustness of Leica stereomicroscopes is We are convinced it will exceed your expectations, as never before have legendary, like any high-tech product, the Leica M series requires a certain we applied our decades of experience in the areas of optics, mechanical degree of care and attention. Therefore, we recommend that you read engineering and ergonomics in such an uncompromising
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A Step Towards Infinity Ever since their introduction by Horatio S. Gree- nough, stereomicroscopes have worked accord- ing to the optical principles based primarily on Ernst Abbe's research. For over a century, ingenious optics designers and engineers have worked to push magnification, resolution and image fidelity to the limit permitted by optics. In doing so, they have always been constrained by the interrelation between three factors: the higher a microscope's resolution, the lower the
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The Electronics: Comfort, Convenience and Safety for your Experiments Never before have electronics been used as Fewer cables extensively in a Leica series as in the new M A large part of the cables have been routed in series lineup. Optics carrier, tripod, base and the interior of the column. Data are also trans- illuminator are all connected using electrical ferred via the interface between the column, contacts—which provides a number of advan- the optics carrier and the carrier. The direct
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Maximum Compatibility Leica engineers were careful to ensure that the Tubes new Leica M series—like its predecessors— The interface between the optics carrier and remains compatible with existing series. This the tube has remained the same, so exist- means that objectives, bases, tubes and so on ing tubes fit the new M series. The new tubes can be reused. are designed for eyepieces with field number 23, while the predecessor models were only Objectives designed for field number 21, resulting
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On We Go If your new Leica microscope has already If, on the other hand, you are assembling the been assembled and commissioned by your microscope yourself, continue with the "Assem- Leica consultant, click here to skip through bly" chapter, which begins on page 19. the installation instructions and go directly to the Quick Start Guide on page 28. Leica M series User Manual 18
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Assembly Leica M series User Manual 19
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Base and Focusing Column The first step is to connect the focusing column Assembling the column adapter Assembling the focusing drive of the M series to the corresponding base. 1. Securely install the column adapter on the 2. Securely screw the focusing column to the column using the four included screws. base using the six included screws. Tools used ★ Allen key, 3 mm Leica M series User Manual 20
