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™
Opsys MW Microplate Washer
User’s Guide
IMPORTANT
Please read carefully before using the Opsys MW
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Part No. 91000051, Revision B This manual is published by DYNEX Technologies, Inc. Questions or comments regarding the content of this manual can be directed to the address below or to your DYNEX Technologies representative. DYNEX Technologies 14340 Sullyfield Circle Chantilly, VA 20151-1683 USA Tel. (703) 631-7800 (800) 288-2354 Fax. (703) 803-1441 TM OPSYS MW is a trademark of DYNEX Technologies, Inc. © 2006 This document is the copyright of DY
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Warranty and Special Provisions Limited Warranty DYNEX Technologies products are fully guaranteed for one year against defects in parts, materials, and workmanship. Defective parts and materials will be replaced or, at the discretion of DYNEX Technologies, repaired at no charge for a period of one year and labor required for such replacement or repair will be provided at no charge for a period of one year, provided that the products are utilized and maintained in accordance with the in
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Table of Contents Table of Contents About this Manual iii Chapter 1 Introduction 1 Description 1 Programming 2 Wash Head 3 Wash Head Positions 4 Wash Fluid 5 Waste 5 Features 7 Specifications 9 Warning Labels 11 Chapter 2 Installation 13 Unpacking 13 Materials Provided 13 Hardware Components 15 Positioning the Instrument 18 Installing the Wash Head 19 Installing the Wash Container 20 Installing the Waste Container 21 Turning on the System 22 Chapter 3 Configuration 23
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Table of Contents Cleaning and Decontamination 69 Cleaning the Containers 70 Replacing the Tubing 71 Pump Calibration 74 Chapter 7 Troubleshooting 77 Operational Problems 77 Chapter 8 Service 81 Service Procedures 81 Spare Parts 82 Returning a Washer for Service 83 Contact DYNEX Technologies 85 Index 86 ii Opsys MW™ User’s Guide
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About this Manual About this Manual This manual has been written for laboratory technicians and provides detailed instructions for using the Opsys MW™ system. This manual gives you the information needed to: • Review safety precautions. • Install the Opsys MW™ system. • Understand the Opsys MW™ menus. • Set the Opsys MW™ system up to suit your exact needs. • Create or modify wash protocols. • Run wash protocols using the Opsys MW™ system. • Perform required preventive maintenance.
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Description Chapter 1 Introduction The Opsys MW™ is a microprocessor-controlled microplate washing system that performs wash protocols that are defined by the user. It is designed to wash all of the wells in one column or one row of a 96-well plate at once. The washing protocol can be programmed so that all of the columns (or rows) are washed in the same manner, or different wash cycles can be applied to specified columns (or rows) on the plate. Description The Opsys MW Microplate Wa
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Description Programming All wash protocols on the Opsys MW™ Microplate Washer are user-defined. Up to 40 wash protocols can be contained on the system. Note: Memory limitations may restrict the size and complexity of an individual wash protocol. In addition, the system can be configured with up to ten different plate types so that the wash head positions for each plate type can be specified. Programming of a new wash protocol (or modifying an existing one) and configuration of the sys
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Description Wash Head The wash head on the Opsys MW™ Microplate Washer contains multiple sets of dual wash pins. Each set of dual wash pins consists of a dispense pin and an aspirate pin that are closely spaced so that fluid can be aspirated from and dispensed into a well simultaneously. The following wash heads can be fitted on the Opsys MW™: Wash Head Description 1 x 8 Eight sets of dual wash pins for washing a 96-well microplate one column (8 wells) at a time. 1 x 12
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Description Wash Head Positions The vertical positions that the wash head can assume are described below. Each wash head position can be specified by the user to within 0.1 mm. Note: Procedures for specifying wash head positions for various plate types are described on page 30. Wash Head Position Description Dispense Height Positions the aspiration pins slightly below the top of the well so that fluid can be dispensed into the well up to a certain level before it is a
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Description Wash Fluid A single wash fluid is used on the Opsys MW™ Microplate Washer. The wash fluid is contained in the Wash Container and is dispensed into the wells whenever a Dispense or Fill operation is specified in the wash protocol. In addition, wash fluid is dispensed into the purge tray during a Purge operation. Dispensing of the fluid from the Wash Container is controlled by a pump attached to the back of the Opsys MW™ Microplate Washer and a dispense valve located near th
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Features Features The Opsys MW™ system has a number of performance and convenience features. These are summarised below: • Small footprint • Up to 40 wash protocols can be programmed • Up to ten plate types can be programmed • Two wash head configurations (1 x 8 and 1 x 12) • On-board self diagnostics • SHOW function allows visual verification of wash head height settings • High-level alarm on Waste Container Opsys MW™ User’s Guide 7
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Specifications Specifications Dimensions and Weight Length 49 cm (19.3 in) Width 38 cm (15.0 in) Height 19 cm (7.5 in) Weight 9.1 kg (20 lbs) Operation Dispense Range 50 – 999 µL Dispense Precision 5% CV (with 300 µL distilled water) Residual Aspirate Volumes < 1 µL for U and V bottom plates < 5 µL for flat bottom plates Washing Time Typical 5 cycle wash: 5 min 30 sec Power < 100 W Display 2 by 20 character back lit LCD Keypad 21 key tactile membrane Output Display, Printer, RS
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Specifications Input Ranges Assay Programming Assay Name Up to 10 characters Password Up to 8 characters Move: Start Strip 1 - 8 for 1 x 8 heads and 1 - 12 for 1 x 12 heads (Increment: 1) Move: End Strip 1 - 8 for 1 x 8 heads and 1 - 12 for 1 x 12 heads (Increment: 1) Move: #Cycles 1 - 9 (Increment: 1) Aspirate: Cycles 1 - 9 (Increment: 1) Dispense 50 - 999 (Increment: 1 µL) Purge 50 - 9999 (Increment: 1 µL) Fill 50 - 300 (Increment: 1 µL) Soak 1 - 999 (Increment: 1 second) Plate
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Warning Labels Warning Labels The Opsys MW™ or its components may contain certain labels that that either warn the user of a hazard or note an electrical connection. The labels that may be used on the Opsys MW™ system are described below. Label Description Alternating current is present. (English) Caution symbol. Refer to the Routine Maintenance chapter. (French) Attention (voir documents d’accompagnement). (English) Caution, hot surface. (French) A
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