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P H A R M A C I A B I O T E C H
®
MultiTemp III
User Manual
18-1106-33
Edition AD
A
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Important user information Reading this entire manual Warranty and Liability Pharmacia Biotech AB guarantees that is necessary for full under- the product delivered has been standing and safe use of thoroughly tested to ensure that it this product. meets its published specifications. The warranty included in the conditions of delivery is valid only if the product has Should you have any comments on been installed and used according to this manual, we will be pleased to the instructions supplied
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Contents 1. Introduction..................................................................... 3 2. Safety information ........................................................... 3 2.1 Safety precautions................................................. 3 2.2 Safety standards.................................................... 4 2.3 Safety features ...................................................... 5 3. Installation....................................................................... 6 3.1 U
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2
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1. Introduction and 2. Safety information 1. Introduction ® MultiTemp III circulates constant temperature liquid to external electrophoresis equipment. The liquid temperature range is -10 to +90 °C. The unit contains KLEA 134a*, an ozone friendly HFC-type coolant. MultiTemp III both supplies coolant for high voltage techni- ques and provides temperature control at higher temperature (e.g. 60 °C) when performing DNA sequencing. When used ® with equipment such as the Pharmacia Biotech Multiphor
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1. Introduction and 2. Safety information 7. Do not use the circulator beyond its specifications or for other than its intended use. 8. Do not touch the stainless steel bath cover and tubings when the unit is used at a high working temperature. Under these conditions, the surface temperature can rise up to approximately 70 °C. MultiTemp III is designed in accordance with: 2.2 Safety standards • Protection class 1 according to IEC 536: all conductive parts that might become accessible are conn
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1. Introduction and 2. Safety information General cut-off 2.3 Safety The heater, the compressor and the circulation pump can be features switched off by a relay built into the unit. This general cut- off will be activated if a malfunction is detected by the safety sensors. See 7.4, Description of Safety Features. Protection against over-heating A built-in temperature limit switch protects against uncon- trolled heating of the bath liquid by activating the general cut-off if this limit is exceed
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3. Installation 3. Installation 1. Check the contents against the packing list supplied. Inspect 3.1 Unpacking for any damage that may have occurred during transit. Report and any damage immediately to your local Pharmacia Biotech installation representative and to the transport company concerned. 2. Place MultiTemp III in its working location, ensuring ade- quate space for ventilation of air through the grids on the sides (air outlet) and rear (air inlet) of the unit. 3. Allow MultiTemp III t
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3. Installation Silicone tubing (inner diameter 8 mm) can be used over the 3.2. Tubing entire working temperature range of the unit. PVC tubing connec- can only be used in the temperature range of 10 to 60 °C. tions 1. Connect the inlet and outlet tubing to the nozzles on the rear of the circulator (Fig. 1). 2. Connect the other ends to the electrophoresis unit. The nozzles have an outer diameter of 9.5 mm. To ensure safe tubing connections, secure all tubing with hose clamps. When MultiTemp I
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3. Installation liquid pour into a large vessel using hydrostatic pressure. The bath contains 3 litres of liquid, plus the volume in tubing and connected equipment. The last millilitres can be removed by opening the screw on the front of the unit, (see Fig. 1). Warning! Do not drain hot water. Use protective gloves and clothing when draining the bath liquid. Compare the local mains voltage and frequency with the spe- 3.5 Mains cifications written on the label on the rear of the unit before conn
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4. Operation 4. Operation 4.1 Start-up 9 10 8 7 6 3 4 1 2 5 Fig. 2. Front panel of MultiTemp III. 1. Mode selector 6. Mains switch 2. Set value down 7. Control lamp cooling on/off 3. Set value up 8. Display 4. Enter 9. Heater control sign 5. Reset 10. Display Before switching on, confirm that the outlet and inlet nozzles are either firmly connected to external equipment or to each other and that the tubing is secured with hose clamps. Switch the power on at the mains switch. The version number o
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4. Operation the external system and the level in the bath will fall. Add more liquid until the level is once again 2 cm below the top plate. If the safety circuit is triggered, please top up with more liqu- id and restart the unit using . Repeat this action if necessary. Note: Check the liquid level after the unit has been in use for a while. When MultiTemp III is switched on, the compressor starts and the heater will be activated if the set temperature is grea- ter then the actual temperature
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4. Operation 2. Use or to set the maximum allowed set tempe- rature. The factory pre-set value is 90.0 °C. 3. Confirm with before the display returns to the actu- al temperature value. Note: The new value is not saved until has been pres- sed. 1. Press to select the S mode. 4.4 Tempera- ture setting 2. Use or to set the working temperature. 3. Confirm with before the display automatically returns to the actual temperature value.
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4. Operation 5. Confirm with before the display returns to the actual temperature value. Note: The new value is not saved until has been pres- sed. 12
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5. Trouble shooting 5. Trouble shooting 5.1 Error messages Message on display Cause Remedy The bath temperature has exceeded Pour in more liquid. the safety temperature limit. Too low water level. Pour in more liquid. The winding of the pump motor is too hot. Check pump and wait a few minutes until the motor temperature has dropped. If this fault occurs often, return the unit for service. The temperature sensor is broken. The sensor must be exchanged by service personnel. The bath liquid has c
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6. Maintenance 6. Maintenance Warning! Always disconnect MultiTemp III from the mains 6.1 General (Pull out the mains plug) before maintenance and/or service. Service should be carried out by authorised personnel only. To maintain efficient operation of the compressor cooling unit, have the air grid and liquifier (heat exchanger) checked and cleaned by service personnel two to four times a year, depending on the amount of dust in the air. Always ensure the liquid bath has sufficient circulating
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6. Maintenance The circuit breaker (thermal fuses) for MultiTemp III are loca- 6.2 Fuses ted on the rear of the unit. Note: If the circuit breaker has been triggered, there may be some delay before it can be released. Please push the circuit breaker button only; do not pull. PUSH Ratings for the circuit breaker: for 198 to 253 V version two circuit breakers 2 x 8 Amps for 95 to 132 V version one circuit breaker 1 x 13 Amps Fuses for the electronics located inside the unit must be repla- ced by
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7. Detailed Description 7. Detailed Description MultiTemp III regulates temperature by balancing the effects 7.1 General of constant cooling and controlled pulses of heating. operating Constant cooling is provided by a built-in unit that includes a principle compressor, an air cooled liquifier and an evaporator (a coo- ling coil as heat exchanger) located in the chamber. This coo- ling unit operates at temperatures up to 68.0 °C. Beyond this temperature, the compressor automatically switches of
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7. Detailed Description Mains Switch. Starts the cooling compressor and the pump motor that circulates the temperature controlled liquid. Activates the heater if the temperature set is greater than the actual temperature. Change the temperature value. Values are changed in an accelerating manner when either key is held down. Changes between showing the actual temperature and the various program modes. Actual temperature mode; shows the actual temperature in the bath. S mode; sets the working te
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7. Detailed Description General cut-off 7.4 Description The heater, the compressor and the circulation pump can be of safety switched off by a relay built into the unit. This general cut- features off will be activated if a malfunction is detected by the safety sensors. When the general cut-off switch is activated, the circulator can only be restarted after the cause of the indicated fault has been eliminated. The display will show when this has occur- red. The circulator can then be re-started
