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LeicaDMI3000B
LeicaDMI4000B
LeicaDMI6000B
Operating Manual
Bedienungsanleitung
MICROSYSTEMS
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Published May 2005 by/ Herausgegeben Mai 2005 von: Leica Microsystems Wetzlar GmbH Ernst-Leitz-Straße D-35578 Wetzlar (Germany) Responsible for contents/ Verantwortlich für den Inhalt: Bernard Kleine (Marketing CMS, Life Science Research Microscopy, Product Management) Holger Grasse (Safety Officer according to MPG §30/ Sicherheitsbeauftragter nach MPG §30) In case of questions, please contact the hotline/ Bei Fragen wenden Sie sich bitte an die Hotline: Phone/Tel. +49 (0) 64 41-29 22 86 Fax +49
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Leica DMI3000B Leica DMI4000B Leica DMI6000B Operating Manual 3
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Copyrights Copyrights All rights to this documentation are held by Leica Microsystems Wetzlar GmbH. Reproduc- tion of text or illustrations (in whole or in part) by print, photocopy, microfilm or other method (in- cluding electronic systems) is not allowed with- out express written permission from Leica Microsystems Wetzlar GmbH. The term “Windows” may appear in the follow- ing text without further identification. It is, how- ever, a registered trademark of Microsoft Corpo- ration. The names of
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Contents Contents 1. Important Notes about this Manual ...... 7 6.11 Installation of Lamp Housing Mount and Mirror Housing ................................... 42 2. Intended Purpose of the Microscope ... 8 6.12 Installation and Replacement of Incident-Light Lamps............................ 44 3. Safety Notes ............................................... 9 6.13 Equipping the 3.1 General Safety Notes ............................... 9 Incident Light Turret Disk ......................... 48 3.2 E
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Contents 8. Operation .................................................... 74 9. Troubleshooting ......................................... 91 8.1 Switching On .............................................. 74 8.2. Contrast Methods ...................................... 76 10. Care of the Microscope ........................... 95 8.2.1 Bright Field (TL) ............................... 76 10.1 Dust Cover .................................................. 95 8.2.2 Phase Contrast (TL) ..............
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1. Important Notes about this Manual 1. Important Notes about this Manual Caution! This operating manual contains important in- This operating manual is an essential com- structions and information for the operational ponent of the microscope, and must be read safety and maintenance of the microscope and carefully before the microscope is assem- accessories. It must therefore be kept safely for bled, put into operation or used. future reference. A separate manual is available on CD-ROM cov- erin
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2. Intended Purpose of the Microscope 2. Intended Purpose of the Microscope The microscopes of the Leica DMI series cov- The above-named microscope complies with ered in this manual are designed for biological the Council Directive 98/79/EEC concerning in routine and research applications. This includes vitro diagnostics. They also conform to the the examination of samples taken from the hu- Council Directives 73/23/EEC concerning electri- man body with a view to providing information cal appara
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3. Safety Notes 3. Safety Notes 3.1 General Safety Notes Caution! This safety class 1 device is constructed and tested in accordance with The devices and accessories described in EN 61010-2-101:2002, this operating manual have been tested for EN 61010-1:2001, safety and potential hazards. IEC 1010-1:2001, The responsible Leica affiliate or the main Safety regulations for electrical measuring, con- plant in Wetzlar must be consulted when- trol, and laboratory devices. ever the device is altere
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3. Safety Notes 3.2 Electrical Safety Caution! General specifications Leica CTR4000, CTR6000 and CTR6500 electron- Power plugs may only be plugged into an ics boxes outlet equipped with a grounding contact. For indoor use only. Do not interfere with the grounding function Supply voltage: 90–250 V~ by using an extension cord without a ground Frequency: 50–60 Hz wire. Any interruption of the ground wire in- Power input: max. 290 VA side or outside of the device, or release of Fuses: T6.3 A the gro
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3. Safety Notes Caution! The microscope’s electrical accessory com- ponents are not protected against water. Water can cause electric shock. Caution! Protect the microscope from excessive tem- perature fluctuations. Such fluctuations can lead to the accumulation of condensation, which can damage the electrical and optical components. Ambient temperature: 15–35°C. Caution! Before exchanging the fuses or lamps, be absolutely certain to switch off the main power switch and remove the power cable. 1
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4. Overview of the Instrument 4. Overview of the Leica DMI Series 4.1 Specifications Contrast Methods Leica DMI Series • transmitted light (DL): BF, DF, PH, DIC, Pol • intermediate pupil: IMC (integrated modulation contrast) IPH (Integrated phase contrast) Leica DMI4000 B and DMI6000 B • incident light (IL): Fluo • combination (DL/IL): Fluo/DIC, Fluo/PH Leica DMI Series Transmitted Light Axis For the Leica DMI3000 B, a manual version of this illumination arm is always a component of the stand. •
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4. Overview of the Instrument Incident light axis Leica DMI4000 B and Leica DMI6000 B • automatic Illumination Manager (aperture, field diaphragm, intensity, process switching) • motorized shutter (switching speed < 50 ms) • lamp housing mount for up to 3 interchangeable light sources • motorized 6-place filter turret • Fluorescence Intensity Manager (FIM) (reduction of incident illumination intensity) • mechanical booster lens for central boosting of fluorescence or uniform distribution • motor
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4. Overview of the Instrument Objective Turret Leica DMI4000 B • manual and coded • 6x for objectives with M25 thread and 45mm parfocal distance • for DIC: motorized or manual/coded Wollaston prism carousel Leica DMI3000 B • manual • 6x for objectives with M25 thread and 45 mm parfocal distance • for DIC: manual Wollaston prism carousel Leica DMI Series Stages Fixed regular stages • Ceramic-coated stage plate (248 mm x 204 mm) • heating stage plate (3°C above room temperature to 60°C) (248 x
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4. Overview of the Instrument Condensers Leica DMI4000 B and Leica DMI6000 B (identical for Leica DMI3000 B, but manual) • motorized and coded or manual and coded • motorized or manual aperture diaphragm • contrast methods: BF, DF, PH, DIC, Pol, IMC, IPH • automatic method switching • condenser turret with 7 positions for contrast methods •2 condenser housings (S1-S28 and S70) • condenser heads: S1/1.4 oil, S1/0.9 dry, S23/0.53, S28/0.55 • condenser heads can be swung out • condenser S70 with ad
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4. Overview of the Instrument Observation ports Leica DMI3000 B (a manual side port is a standard feature of the Leica DMI3000B stand) • manual • left side port (80% transmission) Controls Leica DMI4000 B and Leica DMI6000 B •7 fixed control buttons for illumination and apertures •7 variable function buttons behind the focus controls •3 fixed control buttons for focus stops (Leica DMI6000 B only) •2 focus hand wheels •7 buttons for fluorescence cubes and shutters •4 buttons for magnification cha
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4. Overview of the Instrument Interfaces Leica DMI4000 B and Leica DMI6000 B •2 x RS232C •2 x USB •4 x external/internal peripherals • CTR boxes • SmartMove Software tools Leica DMI4000 B and Leica DMI6000 B TM • Leica Application Suite (LAS) for Windows 2000, XP with plug-ins for: • microscope and camera configuration • microscope and camera control • image acquisition 17
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4. Overview of the Instrument 18 17 1 16 2 15 3 14 4 5 19 13 12 11 10 9 8 7 6 Fig. 1 Left side, Leica DMI4000 B and DMI6000 B 1 Eyepiece 11 Variable function buttons 2 Eyepiece tube 12 Left side port 3 Top port 13 Booster lens (fluorescence microscopes only) 4 Intermediate pupil interface 14 Lamp mount (fluorescence microscopes only) 5 LeicaScreen 15 Condenser head 6 Light intensity 16 Condenser base 7 Field diaphragm 17 Field diaphragm 8 TL/IL switching 18 Transmitted-light lamp housing 9 Apert
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4. Overview of the Instrument 12 11 4 5 12 3 678910 Fig. 2 Right side Leica DMI4000B and DMI6000 B 1 E-Focus buttons (Leica DMI6000 B only) 7 Analyzer slot 2 Focus wheel (motorized Leica DMI6000 B, 8 Centering window (fluorescence microscopes only) manual (fine) Leica DMI4000 B) 9 Field diaphragm centering 3 Variable function buttons (fluorescence microscopes only) 4 Opener for drawer (fluorescence microscopes only) 10 Incident-light lamp housing (fluorescence microscopes 5 Drawer (fluorescence
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4. Overview of the Instrument 5 4 6 3 2 1 Fig. 3 Front view Leica DMI4000 B and Leica DMI6000 B 1 LeicaScreen 2 Front control panel 3 Port switching 4 Top port 5 Manual transmitted-light filters 6 Bertrand lens centering 20
